Involvement of actin microfilaments in the transcription/replication of human parainfluenza virus type 3: Possible role of actin in other viruses

1999 ◽  
Vol 47 (2) ◽  
pp. 114-123 ◽  
Author(s):  
Bishnu P. De ◽  
Amiya K. Banerjee
Keyword(s):  
Virus Type ◽  
Parainfluenza Virus ◽  
Actin Microfilaments ◽  
Human Parainfluenza Virus ◽  
Type 3

scholarly journals Role of Heparan Sulfate in Human Parainfluenza Virus Type 3 Infection

Virology ◽  
2002 ◽  
Vol 298 (1) ◽  
pp. 73-83 ◽  
Author(s):  
Santanu Bose ◽  
Amiya K. Banerjee
Keyword(s):  
Heparan Sulfate ◽  
Virus Type ◽  
Parainfluenza Virus ◽  
Human Parainfluenza Virus ◽  
Type 3

Role of N-linked glycosylation of the human parainfluenza virus type 3 hemagglutinin-neuraminidase protein

2013 ◽  
Vol 174 (1-2) ◽  
pp. 137-147 ◽  
Author(s):  
Fu-lu Chu ◽  
Hong-ling Wen ◽  
Gui-hua Hou ◽  
Bin Lin ◽  
Wen-qiang Zhang ◽  
...  
Keyword(s):  
Virus Type ◽  
Parainfluenza Virus ◽  
Human Parainfluenza Virus ◽  
Type 3

scholarly journals Polarity of Human Parainfluenza Virus Type 3 Infection in Polarized Human Lung Epithelial A549 Cells: Role of Microfilament and Microtubule

2001 ◽  
Vol 75 (4) ◽  
pp. 1984-1989 ◽  
Author(s):  
Santanu Bose ◽  
Achut Malur ◽  
Amiya K. Banerjee
Keyword(s):  
Virus Type ◽  
Human Lung ◽  
Virus Entry ◽  
A549 Cells ◽  
Parainfluenza Virus ◽  
Type Ii ◽  
Lung Epithelial ◽  
Human Parainfluenza Virus ◽  
Type 3

ABSTRACT Human parainfluenza virus type 3 (HPIV-3) is an airborne pathogen that infects the epithelial cells of the respiratory tract. In the present study we investigated the interaction of HPIV-3 with the type II alveolar human lung polarized epithelial A549 cells. Although HPIV-3 entry and budding were bidirectional from both the apical and the basolateral domains, HPIV-3 exhibited preferential entry and release from the apical pole. While disruption of the cellular actin microfilament and microtubule by cytochalasin D and nocodazole, respectively, had no effect on virus entry, disruption of the microtubule but not the microfilament inhibited HPIV-3 release.

scholarly journals Involvement of Actin Microfilaments in the Replication of Human Parainfluenza Virus Type 3

1998 ◽  
Vol 72 (4) ◽  
pp. 2655-2662 ◽  
Author(s):  
Sanhita Gupta ◽  
Bishnu P. De ◽  
Judith A. Drazba ◽  
Amiya K. Banerjee
Keyword(s):  
Virus Type ◽  
Parainfluenza Virus ◽  
Actin Microfilaments ◽  
Viral Mrnas ◽  
Polymeric Form ◽  
Genomic Rnas ◽  
Human Parainfluenza Virus ◽  
Type 3

ABSTRACT Several studies indicate that paramyxoviruses require a specific cellular factor(s) for transcription of their genomic RNAs. We previously reported that the cellular cytoskeletal protein actin, in its polymeric form, participates in the transcription of human parainfluenza virus type 3 (HPIV3) in vitro. In the present study, we investigated the role of the polymeric form of actin, i.e., the actin microfilaments of the cytoskeletal framework, in the reproduction of HPIV3 in vivo. Pulse-chase labeling analyses indicate that the viral nucleocapsid-associated proteins, NP and P, are present predominantly in the cytoskeletal framework during infection. By in situ hybridization, we found that viral mRNAs and genomic RNA were synthesized from the nucleocapsids that were bound to the cytoskeletal framework. Double immunofluorescent labeling and confocal microscopy of the cytoarchitecture revealed that the viral nucleocapsids are specifically localized on the actin microfilaments. Treatment of cells with the actin-depolymerizing agent, cytochalasin D, resulted in the inhibition of viral RNA synthesis and ribonucleoprotein accumulation. These results strongly suggest that actin microfilaments play an important role in the replication of HPIV3.

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